A sensitive, simple and selective spectrofluorimetric method was developed for the determination of pramipexole HCl (PPX) in pharmaceutical dosage forms. The method is based on the reaction between the drug and 4-chloro-7-nitrobenzodioxazole (NBD-Cl) in borate buffer of pH 8.5 to yield a highly fluorescent derivative that is measured at 533 nm after excitation at 464 nm. The calibration curve was linear over the concentration range of 100–1000 ng/mL for standard drug solution. The limit of detection and limit of quantitation was found to be 21.67 ng/ml, and 65.685 ng/ml, respectively. The recovery range of PPX was found to be 99.54 to 101.02%. The method was successfully applied to the determination of PPX in pharmaceutical formulations as well as in-vitro dissolution studies of PPX tablets.
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